Dr. Linda Rinaman

Dr. Linda Rinaman
Stem Field
Title of Research
Central Neural Circuits for Interoceptive Control of Stress Responses and Motivated Behavior
Description of Research Area

The ability of animals to mount adaptive responses to emotional and physiological stress is mediated by central neural pathways that control neuroendocrine secretion, autonomic function, and motivated behavior. The long-term objective of research in the Rinaman lab is to characterize the functional multisynaptic organization of these neural systems, with a focus on circuits that relay visceral sensory (interoceptive) feedback to the brainstem, hypothalamus, and limbic forebrain. Neuroanatomical, physiological, and behavioral techniques are applied to probe these circuits in transgenic and wildtype laboratory rodents, offering unique opportunities to test hypotheses about brain structure-function relationships. Currently funded projects focus on central noradrenergic and glucagon-like peptide 1 (GLP1) neural signaling pathways arising from caudal brainstem. Some ongoing studies analyze stimulus-induced expression of the immediate-early protooncogene c-fos (a marker of neural activation) after animals are exposed to various stress- and anxiety-provoking challenges. Analysis of c-fos expression is combined with retrograde labeling of central neural pathways and immunocytochemical or in situ hybridization (RNAscope)-based detection of neurotransmitter chemicals or mRNA to characterize the axonal projections and phenotypes of stimulus-activated neurons. Other projects use adeno-associated viral (AAV) vectors and engineered neurotropic alpha herpes viruses for condtional transneuronal tracing of multisynaptic neural circuits. Transgenic cre-driver mice and rats also are used for conditional viral-based manipulation of neural activity using DREADDs and optogenetics. Sophisticated microscopic approaches (including CLARITY-based confocal and lightsheet microscopy) enable high-level visualization and mapping of anatomical circuits.

Special Research & Career Skills

Postdoctoral fellows in the Rinaman lab can expect to gain or develop expertise in all of the technical approaches listed above. Fellows meet at least once a week with Dr. Rinaman to review plans and progress, including review of their individual development plan (IDP). These meetings are used to develop/adjust experiments, review published literature, and discuss data analysis and interpretation. Fellows are encouraged (and financially supported) to present their work at national and international conferences. For training in oral presentation skills, Fellows present their data several times a year and receive feedback at the weekly Rinaman lab meeting and other local venues (e.g., monthly Ingestive Behavior multi-faculty meeting, annual FSU Postdoc Research Conference). Fellows are encouraged to prepare a seminal review paper on their own research area of interest as they are developing their experimental project in the Rinaman lab; training in scientific writing is achieved through mutual editing of research abstracts and manuscripts. Fellows also are expected to apply for individual research funding through fellowships and grant programs, with assistance and training provided by Dr. Rinaman.