My laboratory is interested in the organization and maintenance of chromatin at the human inactive X chromosome. We use cutting edge genome engineering approaches as well as molecular genomic techniques (high throughout and non-high throughput, including qPCR, RNA-Seq and ChIP-Seq), fluorescence microscopy and cell biology to address fundamental questions related to epigenetics and chromatin biology.
Current projects focus on: (A) Investigating the roles of specific DNA sequences on the X chromosome and how they are epigenetically regulated to perform important roles in maintaining the 3-dimensional (3D) and heterochromatin organization of the human inactive X chromosome (Xi), and (B) investigating the role of long noncoding RNAs and heterochromatin proteins in maintaining the transcriptional state and the 3D and chromatin organization of the Xi.
Training will be provided in the following areas:
- Proposal preparation skills
- Manuscript writing
- Networking
- Guidance with presentation skills (poster and talks)
- Fluorescence microscopy
- Genome engineering
- Genomics